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And, prostate cells were pretreated with RSL3 (MedChemExpress, China) at 0.25 M, 0.5 M, 1 M, 2 M or 4 M for 24 h to activate ferroptosis, isosilybin B (MedChemExpress, China) at 20 M, 40 M, 60 M, 80 M or 100 M for 24 h to activate apoptosis, Rapamycin (Rapa, MedChemExpress, China) at 0.5 nM, 1 nM, 2 nM, 4 nM or 8 nM for 24 h to activate autophagy, Chloroquine (CQ, MedChemExpress, China) at 10 M, 20 M, 30 M, 40 M or 50 M for 24 h to inhibit autophagy, and GSK621 (MedChemExpress, China) at 5 M, 10 M, 20 M, 30 M or 40 M for 24 h to activate AMPK

Patients with a history of diabetic retinopathy should be monitored for progression of diabetic retinopathy during treatment