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Screening is not gatekeeping

pHLA-I complexes were immunoprecipitated from cell lysates, and peptides were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS)

For 96-well plates, 10 L of prepared reagent (1:50 ratio of monochlorobimane (MCB) to Tris assay buffer, per manufacturers instructions) was added to wells containing 100 L of culture media and incubated for 30 min at 37 C, washed with PBS, and imaged using an Olympus IX70 microscope with a DAPI filter (Excitation/Emission: 358 nm/461 nm)