The preclinical data place GHK-Cus influence primarily in the proliferation and remodeling phases recruiting and activating fibroblasts, feeding matrix synthesis, supporting new-vessel formation to perfuse the growing tissue, and then enabling proper cross-linking so the new matrix gains strength
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A landmark review published in Biomolecules documenting GHK-Cus regulatory effects on over 4,000 human genes, including significant upregulation of collagen synthesis, anti-inflammatory pathways, and stem cell mobilization established the molecular basis for its clinical use
The peroxidase reaction was performed using 0.05% 3,3-diaminobenzidine, 0.25% nickel sulfate and 0.03% hydrogen peroxide resulting in a black nuclear staining