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The supernatant was then adjusted to pH 4.0 with acetic acid, loaded onto a HiTrap Q HP column that was installed on FPLC KTA purifier system (GE Healthcare) and pre-equilibrated in pH 4.0 water, and eluted with a linear gradient of pH 4.0 water supplemented with 500 mM NaCl

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ELISA results also indicated an activation effect of the CAR-T cells when cocultured with tumor primary cells

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