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The pipette solution for whole-cell recordings was modified to include an intracellular dye (Alexa Fluor 350 hydrazide dye) and contained: 120 mM K-gluconate, 10 mM KCl, 10 mM HEPES, 5 mM EGTA, 1 mM CaCl 2 , 1 mM MgCl 2 and 2 mM MgATP, and 0.03 mM Alexa Fluor 350 hydrazide dye (pH 7.3)

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academic.oup.com Mechanisms of Action in Hormonal Regulation: GLP-1 receptor agonists exert effects on hormonal regulation through interactions with the hypothalamic-pituitary-gonadal axis and metabolic pathways, contributing to improved reproductive health in PCOS

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The more receptors that are bound, the more they are activated, and the better the glycemic response is

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The current challenge thus is of first clarifying what is normal, as an absence of HE does not necessarily equal absence of neuropsychometric abnormality [14], and then determining optimal methods by which early HE can be consistently and accurately distinguished

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