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Confocal images were acquired with a Zeiss inverted LSM 789 laser scanning confocal microscope (Zeiss) using a 100x/1.4 DIC Plan-Apochromat oil immersion objective
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Gudbergsen H, Overgaard A, Henriksen M, Whrens EE, Bliddal H, Christensen R, et al
a low dose of tadalafil such as 5 mg or 10 mg, or who is only marginally responsive to such dose an oral dose of tadalafil could be taken between about one and one-half hours and about 16 hours prior to an IN dose of PT-141
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