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ELISA for Exe4 concentration measurement Exe4 concentration in the culture medium and mice plasma was measured using the Exendin-4 ELISA kit (Abcam) according to the manufactures protocol

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By removing the need for manual reconstitution before use, the pen simplifies preparation and helps maintain consistent handling throughout experimental protocols

Foods to prioritize and foods to avoid Prioritize: fatty fish (salmon, sardines, mackerel), eggs, full-fat dairy if tolerated, avocado, olive oil and coconut oil, beef and lamb, dark meat poultry, nuts and seeds in moderation (watch carb counts), low-starch vegetables (leafy greens, broccoli, cauliflower, zucchini, asparagus), and bone broth for both protein and electrolytes