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(b) positive reverse transcription real-time polymerase chain reaction findings (rRT-PCR)

DOX was acquired as an injection-grade vial (50 mg/50 mL, Mylan, Italy), SP as tablets (the active ingredient is included in 500 mg each pill, Puritans Pride Company, Inc., USA), and L-car as injection-grade ampoules (1 mg/5 mL/5 ampoules, Mepaco, Cairo)

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The GST activity is determined by measuring the rate of produced conjugation between reduced glutathione and CDNB, which is proportional to the increase in absorbance at 340nm over time (OD 340nm /min)