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Sodium 4-phenylbutyrate (4-PBA) is a low molecular weight fatty acid, that has been used in the treatment of several diseases, including liver injury by ischemia/reperfusion (102) and carbon tetrachloride toxicity (104)

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Imaging and figure preparation Images of hypothalamic cultures were collected on the calcium imaging microscope as described above, and separate cultures were plated onto PhenoPlateTM 96-well microplates (Perkin Elmer), fixed in 4% paraformaldehyde (Thermo Fisher Scientific) for 10 minutes, rinsed three times with Tris-buffered saline containing 0.1% Triton X-100 (TBS-T

Cell Stress