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The practical distinction in experimental design is compartmental

(2) Choline group: mice were maintained on a choline-supplemented diet (1.0% total choline, Trophic Animal Feed High-tech Co., Ltd, China) 2

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As a result, defenses against ROS are activated 14 by an array of nonenzymatic antioxidants [metabolites such as ascorbate (AsA), carotenoids, glutathione (GSH) and proline] and antioxidant enzymes [such as guaiacol peroxidases (GPOX), catalase (CAT), superoxide dismutase (SOD) and AsA-GSH cycle enzymes like glutathione reductase (GR) ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR)], work together for detoxification of ROS 7,8,15,16,17,18,19,20