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Proteins were eluted by adding 3.25 l of 4x Laemmli SDS Loading Buffer to the supernatant and 10 l of 1x Laemmli SDS Loading Buffer and heated at 60 C for 3 min

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H 2 S, for example, can bind to protein metal centres, such as haem moieties in mitochondrial cytochrome c oxidase or haemoglobin (reviewed e.g