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Overexpressing ACMSD reduces the NAD + level by dissipating ACMS from the de novo NAD + synthesis into the side branch pathway for acetyl-CoA production, while inhibiting ACMSD elevates NAD + concentration
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Decant the supernatant and resuspend the washed pellet in 12 ml U-buffer (resuspend in 20 l U-buffer per ml of original culture)
Most of the analytic work in this study was performed on an LCMS system that represents a powerful tool in identifying and characterizing xenobiotic metabolites (Oliveira and Watson 2000)
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