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Immunoblotting To profile protein lipoylation and lysine glutarylation, 0.5 10 6 HeLa cells or 1 10 6 T cells were lysed in SDS loading buffer (2% (wt/vol) SDS, 50 mM Tris (pH 7.4), 150 mM NaCl, 1 mM dithiothreitol, 10% (vol/vol) glycerol and 0.2% (vol/vol) benzonase nuclease (Sigma-Aldrich, E1014)) and heated at 90 C for 5 min

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According to a review published in Natures Signal Transduction and Targeted Therapy, the global peptide therapeutics market grew from $41.44 billion in 2023 to $45.66 billion in 2024, a compound annual growth rate of over 10%, reflecting the rapid expansion of peptide research and clinical applications