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Once reconstituted, maintain refrigeration at 28C and avoid freeze-thaw cycles

G (00:12:32): That's Kenny, Kenny Rogers who is what everyone thinks of when you think of bad male plastic surgery because whoever did his eyes needs to be fired

On day 0, 25 g Nras G12V plasmid and 5 g Sleeping Beauty transposase expression vector were diluted in sterile normal saline to a final volume of 2 ml (~10% of body weight)