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Its prevalence has risen dramatically since 1980, when it was a perplexing and sporadically-observed disease as first reported by Ludwig and colleagues (1), to a current estimated 25% global prevalence.(2) NAFLD represents the leading cause of chronic liver disease and is the second leading indication for liver transplantation in the United States.(3) Its rise parallels the obesity epidemic and is attributed to the concomitant rise in the prevalence of type 2 diabetes and metabolic syndrome
Cells were imaged in a Nikon spinning disk field scanning confocal microscope using a 100X oil objective under TIRF illumination with the following microscope settings using the MetaMorph software: 488 nm excitation laser, exposure time of 150 msec, 10% laser power at a rate of 1 frame per second for 150 frames
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