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Description

cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)

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Deficiency of SELENOO interfered with the chondrogenic differentiation of ATDC5 cells by suppressing the expression of chondrogenic genes SOX9, COLII, and aggrecan and decreasing the activity of alkaline phosphatase 112

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