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*Correspondence: Mizuki Ishiguro, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

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For digestion, trypsin (PRV5111, Promega, Wisconsin, USA), made up in 50 mM TEAB (pH 8), was added at 1:10 trypsin:total protein weight ratio, and the plate was incubated at 37 C on a shaker at 900 rpm for four hours

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