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Use a syringe that matches your insulin concentration (dont mix U-100 insulin and a U-40 syringe, for example)

Binding was fit with the followingquadratic binding equation in Prism (GraphPad): where M = fully unbound signal (baseline signal), Q = fully bound (saturation) signal, P = concentration of fixed species, X = ligand concentration, Y = observed fluorescence intensity, and K = association constant

A total of 100 nM ABHD11-Flag was added to 100 M of the substrates (OGDHc-E2 peptides or succinyl-CoA) in assay buffer (50 mM TrisHCl pH 7.4, 100 mM NaCl) in 1.5 ml Eppendorf tubes for each condition on ice

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