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After washing in PBS, cells were treated with vehicle or 100 nM GLP-1 for 10 minutes at 37C in complete medium before being washed 1x in cold PBS, with all subsequent steps performed on ice

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Yes Total cumulative deposit $1000 Additional Information about Deposit Applicants who receive an offer have 3 business days to either indicate an interest in the program via email and begin the admission process or to decline the offer