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The primers used in the qPCR experiments were: FBI-1, forward sequence: 5'-GCAACATCTGCAAGGTCCGCTT-3', reverse sequence: 5'-TCTTCAGGTCGTAGTTGTGGGC-3'

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When GHK-Cu was injected into these chambers, the harvested tissue contained more collagen and more glycosaminoglycans than saline-control chambers, in a concentration-dependent manner, indicating accelerated and more robust connective-tissue formation

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