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Real-time PCR Total RNA was isolated using the QIAzol Lysis Reagent (Qiagen) according to the manufacturers instructions and diluted in nuclease-free water (Ambion/Life Technologies)

doi: 10.1093/jn/133.6.1921

Proc Natl Acad Sci USA 110(50):48844893 Wojciechowski W, Harris DP, Sprague F, Mousseau B, Makris M, Kusser K, Honjo T, Mohrs K, Mohrs M, Randall T et al (2009) Cytokine-producing effector B cells regulate type 2 immunity to H