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The gene expression level was normalized to the level of GAPDH (Forward: 5-ACCCACTCCTCCACCTTTGA-3 and Reverse 5-TGTTGCTGTAGCCAAATTCGTT-3) and determined using the C T method
The GST-catalyzed GSHCDNB reaction can be followed with AuNPs as colorimetric probes, and one can easily use the A 670 / A 520 ratio to evaluate whether the molecule can inhibit GST activity and compare the inhibition efficiency among different inhibitors
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