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Berberine activates AMPK through a different mechanism than MOTS-c (via inhibition of complex I in mitochondria), so concurrent use may create additive rather than synergistic AMPK activationhigher risk of hypoglycemia without proportional metabolic benefit

418,419,420,421,422,423 It is worth noting, however, that investigations on E/I imbalance have primarily been carried out using animal models, therefore a detailed assessment of the pathophysiology of E/I imbalance contributing to human ASD is warranted

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