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First, we set the ratios (Km, wt:wt) of Tween 80 and Span 20 (T/S) as 1:1, 1:2, 1:3, 2:1, and 2:3, and evaluated the effect of different T/S ratios on the area of the microemulsion region by constructing pseudo-ternary phase diagrams for a water/IPM system