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The MSEL is a developmentally integrated system that assesses language, motor, and perceptual abilities for children aged from birth to 68 months of age ( Behavioral concerns were measured by the Child Behavior Checklist 1-5 Language Development Survey (CBCL)

After centrifugation, the bacterial supernatants were loaded onto a Ni Sepharose High-performance (Cytiva) column, and the proteins were eluted with 500 mM imidazole

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Note, we reported a subset of the SM1 GABA+ data (from 24 ASD and 26 TDC participants) and Thal GABA+ data (from 16 ASD and 9 TDC participants) in our previous work(He et al., 2021)