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Reaction and catalytic mechanism [edit] All family members are capable of catalyzing the hydrolysis of PIP 2 , a phosphatidylinositol at the inner leaflet of the plasma membrane into the two second messengers, inositol trisphosphate (IP 3 ) and diacylglycerol (DAG)

doi: 10.1111/j.1464-5491.2009.02666.x 220 SorliCHarashimaSITsoukasGMUngerJKarsblJDHansenTet al

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When you should take it really depends on how you work out, how fast your body burns fuel and what you are trying to get out of it