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Spiking/recovery tests were performed to determine the optimal dilution factor, particularly for the kits lacking manufacturer-provided serum dilution guidelines [human pro-BDNF DuoSet ELISA kit (#DY3175, R&D Systems) and high-sensitivity pro-BDNF human ELISA kit (#SK00752-09, Aviscera Bioscience)]

However, most current studies rely solely on the EAE mouse model and the LPC-induced localized myelin loss model, which do not fully recapitulate the complex pathological features of MS, particularly its heterogeneity, disease progression, and clinical symptoms

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It will have lost significant potency, leading to inaccurate results, and it may be contaminated with bacteria, posing a safety risk