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GLP-1 Arg 36 replacement with Ala obviously hinders affinity (IC 50 : 0.27 nM4.6 nM) and activity (EC 50 : 2.6 nM7 nM)

The findings demonstrated that the interactions between GLP-1/GLP-1R and HSA-DARPin/HSA persisted even after the amino acid substitution in GLP-1 and the formation of the fusion protein

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