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Exogenous cystine increases susceptibility of drug-resistant Salmonella to gentamicin by promoting oxidation of glutathione metabolism and imbalance of intracellular redox levels

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Expression and purification of SmTrx SmTrx was expressed, purified, and quantified using methods previously established ( Anaerobic techniques and transient-state conditions Following published protocols ( For each variant the reductive half-reaction was observed using both photomultiplier (PMT) detection at individual wavelengths specific to the observation and using charge coupled device (CCD) detection for all wavelengths between 300 and 800 nm

The optimization of this structural element represents a key step in enhancing laboratory performance and reproducibility