l glutathione immune system NutriDyn L-Glutathione Reduced 250 mg 60

(DMEM/F12, Gibco, Gaithersburg, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco, Gaithersburg, USA) and 1% penicillin/streptomycin (Invitrogen, Life Technologies, USA) in a humidified incubator with 5% CO 2 at 37 C

Swirl or roll gently until fully dissolved the solution should be clear

gBlocks containing a FLAG tag followed by the full coding sequence of human CDKN1A , the N terminus only (amino acids 190), or the C terminus (amino acids 87164) were synthesized by IDT DNA and cloned into lentiviral vectors containing a Tet-inducible promoter (pLenti-CMV-TRE3G-Puro, a gift of Jan Carette) by Gibson assembly

Target the muscle or tissue under the skin