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In their study, human endothelial cells treated with HTY and sulphate metabolites showed reduced reactive oxygen species (ROS) generation and downregulation of adhesion-related genes such as ICAM1, VCAM1, and E-selectin genes [74]

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The washed immunoprecipitates were incubated in 50 L of 1 electrophoresis loading buffer and heated to 100 C for 5 min

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