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Given that proline and hydroxyproline together account for approximately one-third of collagens amino acid content, increased proline biosynthesis is now considered a key metabolic driver of fibrotic remodeling [13,14,15,16]

Its potential benefits stem from its role in intracellular signaling pathways that influence insulin sensitivity and ovarian function mechanisms that underlie many of the outcomes studied in clinical research.7 12 15 17 Although inositol is often associated with PCOS, much of the research points to metabolic signaling as the common thread, with reproductive and fertility-related benefits often building on those effects

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In brief, male mice were deeply anaesthetized with an intraperitoneal injection of 7% chloral hydrate and transcardially perfused with a modified ice-cold artificial cerebrospinal fluid (aCSF) (described below)