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Researchers often report that this is when they notice the first visible changes in problem areas, particularly abdominal fat

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After a 20-min incubation with immunol staining blocking buffer (Beyotime, Shanghai, China), cells were continuously incubated with mouse anti-SMA (1:100, Abcam) and rabbit anti-CD133 (1:100, Abnova) 44 at 4 C overnight