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Body mass data were analyzed separately in young and aged individuals, due to the known differences in body mass between young and aged animals, using two-way ANOVA with group as a between-subjects factor and time (day) as a within-subjects factor

MATERIALS AND METHODS Animals and Surgery Male Sprague-Dawley rats (Taconic derived) weighing 390-450 g were maintained with free access to water and food (Harland Tekland F6 rodent diet, Madison, WI) except the night prior to surgery when food was removed

Initially, you may notice subtle improvements like increased energy, better digestion, or even clearer skin

Acknowledgements The authors wish to thank the anonymous reviewers for their constructive critique