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For immunofluorescence analysis, the sections were heated in a microwave oven in citrate buffer, after cooling, blocked with 5% BSA in PBS and 0.5% (wt/vol) Triton X-100 for 30 min at room temperature, and subsequently incubated with primary antibodies at 4 C overnight and secondary antibodies for 1 h at room temperature, followed by washing and staining with DAPI (Solarbio, S2110)

Physical examinations were performed at screening and weeks 52 and 104

Learn more about how semaglutide compares to other medications: Are you ready to stop taking semaglutide