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Extending the incubation to 72 hours exacerbated these trends, and even GSH levels fell by 33%, and GSSG increased by 81% from the baseline (Supplementary Figure 1g-l)

8.1.3 Network biology approach While pathway analysis highlights the dysregulated processes, network-based approaches extend this by mapping the interaction between genes, proteins, and molecular regulators within complex cellular systems, offering a complete holistic view of system-level changes (287, 288)

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and the 6 questions version (IIEF-6) addressing exclusively penile erectile function, but considering the whole spectrum of erection related aspects, including erection frequency, erection firmness, penetration ability, erection maintenance frequency, erection maintenance ability, and erection confidence [30, 31]