The reason we do not wait until 12-16 weeks is because we cannot be sure a) whether the mother was properly vaccinated and b) whether the puppy/baby received enough immunoglobulins through first milk colostrum (a bigger risk in large litters where pups fight for resources)
Alcohol has been known to mitigate the production of antidiuretic hormone, which is a hormone that acts on the kidney to favor water reabsorption in the kidneys during filtration
This is especially important for those who may have difficulty absorbing nutrients due to digestive issues, certain medical conditions, or simply because of age-related changes
Abbreviations AKT1: RAC-alpha serine/threonine-protein kinase BMI: Body Mass Index CpG: Cytosine-phosphate-Guanine dinucleotide DESeq2: Differential Expression analysis based on the Negative Binomial distribution DNA: Deoxyribonucleic Acid DMSO: Dimethyl Sulfoxide FDR: False Discovery Rate FC: Fold Change GSEA: Gene Set Enrichment Analysis GO: Gene Ontology GRCh38: Genome Reference Consortium Human Build 38 IPA: Ingenuity Pathway Analysis KEGG: Kyoto Encyclopedia of Genes and Genomes LP: Lipedema Patient HC: Healthy Control MTBE: Methyl-t-butyl ether NMR: Nuclear Magnetic Resonance PLS-DA: Partial Least Squares Discriminant Analysis RIN: RNA Integrity Number RNA: Ribonucleic Acid RNA-Seq: RNA Sequencing SD: Standard Deviation SOP: Standard Operating Procedure STAR: Spliced Transcripts Alignment to a Reference TCA: Tricarboxylic Acid (cycle) TSS: Transcription Start Site TSP-d4: 2,2,3,3-d4-trimethylsilyl-propionic acid sodium salt UTR: Untranslated Region VIP: Variable Importance in Projection References Herbst KL, Kahn LA, Iker E, Ehrlich C, Wright T, McHutchison L, Schwartz J, Sleigh M, Donahue PM, Lisson KH, et al
