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To monitor cell viability, the cells were trypsinized, and apoptosis/necrosis induced by pneumococcal infection was evaluated using flow cytometry with an Annexin V/propidium iodide labeling kit (Invitrogen), showing 5% across all time points

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Bai XJ, Hao JT, Zheng RH, et al

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F2-isoprostane (F2-osiprostoglandin, 8-iso-prostoglandin F2 (alpha)) is the oxidized degradation product of arachidonic acid

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