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HETEROLOGOUS EXPRESSION OF AsGGT1, AsGGT2, AND AsGGT3 IN YEAST The coding regions of AsGGT1 , AsGGT2 , and AsGGT3 were amplified by PCR using the cloned cDNA fragments described above, KOD plus DNA polymerase (Toyobo), and the following gene-specific primers: AsGGT1-FKpn3A (5- GGTACC AAAATGAACCAAATGGCGCCGGCTTC-3) and AsGGT1-stop-RXh (5- CTCGAG CTATACACAAGCAGGACTTC CATC-3) for AsGGT1

Transwells were then incubated at 37 C for 30 min, changed to media containing 100 g ml 1 gentamicin to clear the extracellular bacteria and cultured for an extended time

In the future it could be a useful combination therapy with HU (Minniti, 2018) but uptake among patients is still low, one of the reasons is the unpleasant taste

+ Lambs: 1 ml per 5-8 kg body weight per day