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Potentials of ketogenic diet against chronic kidney diseases: pharmacological insights and therapeutic prospects

In animal experiments, the GLP-1RA liraglutide was found to stimulate the secretion of ANP by increasing the levels of Epac2, which is a downstream target of GLP-1R signaling in cardiomyocytes (132), However, this finding seemed to contradict the results of clinical studies in which no increase was observed in the levels of ANP in healthy people and patients with T2MD

After scratching the cells to make a wound, 3 10 9 particles of fluorescently labeled l-EVs were added to the 4 10 6 cells in 200 l of phenol red-free DMEM medium (Gibco, #21063029) containing 10% EV-depleted FBS, and the cells were incubated for 1 h before the observation